Dbfetch
ID GM662856; SV 1; linear; unassigned DNA; PAT; SYN; 7873 BP. XX AC GM662856; XX DT 19-DEC-2008 (Rel. 98, Created) DT 19-DEC-2008 (Rel. 98, Last updated, Version 1) XX DE Sequence 191 from Patent WO2008073275. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RA Damude H.G., He H., Liao D.I., Zhu Q.Q.; RT "Mutant delta-8 desaturase genes engineered by targeted mutagensis and RT their use in making polyunsaturated fatty acids"; RL Patent number WO2008073275-A2/191, 19-JUN-2008. RL E. I. Du Pont De Nemours And Company (US). XX DR MD5; 71693c8cccff636207624f3be9e29d75. XX FH Key Location/Qualifiers FH FT source 1..7873 FT /organism="synthetic construct" FT /mol_type="unassigned DNA" FT /note="Plasmid pKR1010" FT /db_xref="taxon:32630" XX SQ Sequence 7873 BP; 2024 A; 1849 C; 1819 G; 2181 T; 0 other; cgcgccgttc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa 60 ttgtagccgc gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct 120 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 180 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 240 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 300 ctatttttat aggttaatgt catgaccaaa atcccttaac gtgagttttc gttccactga 360 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 420 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 480 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 540 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 600 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 660 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 720 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 780 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 840 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 900 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 960 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 1020 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 1080 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 1140 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 1200 tggccgattc attaatgcag gttgatcgat tcgacatcga tctagtaaca tagatgacac 1260 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat 1320 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat 1380 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc 1440 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac 1500 gcactccttc tttaggtacc tcactattcc tttgccctcg gacgagtgct ggggcgtcgg 1560 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg 1620 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc 1680 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga 1740 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc 1800 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg 1860 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg 1920 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact 1980 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg 2040 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg 2100 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc 2160 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca 2220 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg 2280 caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg 2340 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt 2400 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc 2460 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca 2520 gacgtcgcgg tgagttcagg ctttttcatg gtttaataag aagagaaaag agttcttttg 2580 ttatggctga agtaatagag aaatgagctc gagcgtgtcc tctccaaatg aaatgaactt 2640 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca 2700 gtggagatgt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc 2760 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga 2820 atgatagcct ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact 2880 gtcctttcga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt 2940 atcctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgacattt 3000 ttggagtaga ccagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg 3060 agtcgtaaaa gactctgtat gaactgttcg ccagtcttca cggcgagttc tgttagatcc 3120 tcgatttgaa tcttagactc catgcatggc cttagattca gtaggaacta cctttttaga 3180 gactccaatc tctattactt gccttggttt atgaagcaag ccttgaatcg tccatactgg 3240 aatagtactt ctgatcttga gaaatatgtc tttctctgtg ttcttgatgc aattagtcct 3300 gaatcttttg actgcatctt taaccttctt gggaaggtat ttgatctcct ggagattgtt 3360 actcgggtag atcgtcttga tgagacctgc tgcgtaggcc tctctaacca tctgtgggtc 3420 agcattcttt ctgaaattga agaggctaac cttctcatta tcagtggtga acatagtgtc 3480 gtcaccttca ccttcgaact tccttcctag atcgtaaaga tagaggaaat cgtccattgt 3540 aatctccggg gcaaaggaga tctcttttgg ggctggatca ctgctgggcc ttttggttcc 3600 tagcgtgagc cagtgggctt tttgctttgg tgggcttgtt agggccttag caaagctctt 3660 gggcttgagt tgagcttctc ctttggggat gaagttcaac ctgtctgttt gctgacttgt 3720 tgtgtacgcg tcagctgctg ctcttgcctc tgtaatagtg gcaaatttct tgtgtgcaac 3780 tccgggaacg ccgtttgttg ccgcctttgt acaaccccag tcatcgtata taccggcatg 3840 tggaccgtta tacacaacgt agtagttgat atgagggtgt tgaatacccg attctgctct 3900 gagaggagca actgtgctgt taagctcaga tttttgtggg attggaattg gatcgatctc 3960 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat 4020 aattttgttt aactttaaga aggagatata cccatggaaa agcctgaact caccgcgacg 4080 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 4140 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 4200 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 4260 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 4320 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 4380 gctgttctgc agccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag 4440 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 4500 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 4560 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc 4620 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc 4680 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc 4740 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc 4800 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt 4860 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg 4920 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc 4980 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga 5040 aaccgacgcc ccagcactcg tccgagggca aaggaatagt gaggtacagc ttggatcgat 5100 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa 5160 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 5220 actatatccg gatgatcggg cgcgccgtcg acggatccgt acgagatccg gccggccaga 5280 tcctgcagga gatccaagct tttgatccat gcccttcatt tgccgcttat taattaattt 5340 ggtaacagtc cgtactaatc agttacttat ccttccccca tcataattaa tcttggtagt 5400 ctcgaatgcc acaacactga ctagtctctt ggatcataag aaaaagccaa ggaacaaaag 5460 aagacaaaac acaatgagag tatcctttgc atagcaatgt ctaagttcat aaaattcaaa 5520 caaaaacgca atcacacaca gtggacatca cttatccact agctgatcag gatcgccgcg 5580 tcaagaaaaa aaaactggac cccaaaagcc atgcacaaca acacgtactc acaaaggtgt 5640 caatcgagca gcccaaaaca ttcaccaact caacccatca tgagccctca catttgttgt 5700 ttctaaccca acctcaaact cgtattctct tccgccacct catttttgtt tatttcaaca 5760 cccgtcaaac tgcatgccac cccgtggcca aatgtccatg catgttaaca agacctatga 5820 ctataaatag ctgcaatctc ggcccaggtt ttcatcatca agaaccagtt caatatccta 5880 gtacaccgta ttaaagaatt taagatatac tgcggccgca ccatggaggt ggtgaatgaa 5940 atagtctcaa ttgggcagga agttttaccc aaagttgatt atgcccaact ctggagtgat 6000 gccagtcact gtgaggtgct ttacttgtcc atcgcatttg tcatcttgaa gttcactctt 6060 ggcccccttg gtccaaaagg tcagtctcgt atgaagtttg ttttcaccaa ttacaacctt 6120 ctcatgtcca tttattcgtt gggatcattc ctctcaatgg catatgccat gtacaccatc 6180 ggtgttatgt ctgacaactg cgagaaggct tttgacaaca acgtcttcag gatcaccacg 6240 cagttgttct atttgagcaa gttcctggag tatattgact ccttctattt gccactgatg 6300 ggcaagcctc tgacctggtt gcaattcttc catcatttgg gggcaccgat ggatatgtgg 6360 ctgttctata attaccgaaa tgaagctgtt tggatttttg tgctgttgaa tggtttcatc 6420 cactggatca tgtacggtta ttattggacc agattgatca agctgaagtt ccccatgcca 6480 aaatccctga ttacatcaat gcagatcatt caattcaatg ttggtttcta cattgtctgg 6540 aagtacagga acattccctg ttatcgccaa gatgggatga ggatgtttgg ctggttcttc 6600 aattactttt atgttggcac agtcttgtgt ttgttcttga atttctatgt gcaaacgtat 6660 atcgtcagga agcacaaggg agccaaaaag attcagtgag cggccgcaag tatgaactaa 6720 aatgcatgta ggtgtaagag ctcatggaga gcatggaata ttgtatccga ccatgtaaca 6780 gtataataac tgagctccat ctcacttctt ctatgaataa acaaaggatg ttatgatata 6840 ttaacactct atctatgcac cttattgttc tatgataaat ttcctcttat tattataaat 6900 catctgaatc gtgacggctt atggaatgct tcaaatagta caaaaacaaa tgtgtactat 6960 aagactttct aaacaattct aaccttagca ttgtgaacga gacataagtg ttaagaagac 7020 ataacaatta taatggaaga agtttgtctc catttatata ttatatatta cccacttatg 7080 tattatatta ggatgttaag gagacataac aattataaag agagaagttt gtatccattt 7140 atatattata tactacccat ttatatatta tacttatcca cttatttaat gtctttataa 7200 ggtttgatcc atgatatttc taatatttta gttgatatgt atatgaaagg gtactatttg 7260 aactctctta ctctgtataa aggttggatc atccttaaag tgggtctatt taattttatt 7320 gcttcttaca gataaaaaaa aaattatgag ttggtttgat aaaatattga aggatttaaa 7380 ataataataa ataacatata atatatgtat ataaatttat tataatataa catttatcta 7440 taaaaaagta aatattgtca taaatctata caatcgttta gccttgctgg acgaatctca 7500 attatttaaa cgagagtaaa catatttgac tttttggtta tttaacaaat tattatttaa 7560 cactatatga aatttttttt tttatcagca aagaataaaa ttaaattaag aaggacaatg 7620 gtgtcccaat ccttatacaa ccaacttcca caagaaagtc aagtcagaga caacaaaaaa 7680 acaagcaaag gaaatttttt aatttgagtt gtcttgtttg ctgcataatt tatgcagtaa 7740 aacactacac ataacccttt tagcagtaga gcaatggttg accgtgtgct tagcttcttt 7800 tattttattt ttttatcagc aaagaataaa taaaataaaa tgagacactt cagggatgtt 7860 tcaacaagct tgg 7873 //