Dbfetch
ID GM662856; SV 1; linear; unassigned DNA; PAT; SYN; 7873 BP.
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AC GM662856;
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DT 19-DEC-2008 (Rel. 98, Created)
DT 19-DEC-2008 (Rel. 98, Last updated, Version 1)
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DE Sequence 191 from Patent WO2008073275.
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KW .
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OS synthetic construct
OC other sequences; artificial sequences.
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RN [1]
RA Damude H.G., He H., Liao D.I., Zhu Q.Q.;
RT "Mutant delta-8 desaturase genes engineered by targeted mutagensis and
RT their use in making polyunsaturated fatty acids";
RL Patent number WO2008073275-A2/191, 19-JUN-2008.
RL E. I. Du Pont De Nemours And Company (US).
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DR MD5; 71693c8cccff636207624f3be9e29d75.
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FH Key Location/Qualifiers
FH
FT source 1..7873
FT /organism="synthetic construct"
FT /mol_type="unassigned DNA"
FT /note="Plasmid pKR1010"
FT /db_xref="taxon:32630"
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SQ Sequence 7873 BP; 2024 A; 1849 C; 1819 G; 2181 T; 0 other;
cgcgccgttc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa 60
ttgtagccgc gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct 120
gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 180
gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 240
tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 300
ctatttttat aggttaatgt catgaccaaa atcccttaac gtgagttttc gttccactga 360
gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 420
atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 480
gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 540
gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 600
tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 660
accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 720
ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 780
cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 840
agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 900
ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 960
tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 1020
ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 1080
cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 1140
gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 1200
tggccgattc attaatgcag gttgatcgat tcgacatcga tctagtaaca tagatgacac 1260
cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat 1320
gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat 1380
gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc 1440
ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac 1500
gcactccttc tttaggtacc tcactattcc tttgccctcg gacgagtgct ggggcgtcgg 1560
tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg 1620
gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc 1680
tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga 1740
ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc 1800
tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg 1860
gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg 1920
cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact 1980
tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg 2040
acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg 2100
aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc 2160
gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca 2220
gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg 2280
caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg 2340
gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt 2400
acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc 2460
tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca 2520
gacgtcgcgg tgagttcagg ctttttcatg gtttaataag aagagaaaag agttcttttg 2580
ttatggctga agtaatagag aaatgagctc gagcgtgtcc tctccaaatg aaatgaactt 2640
ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca 2700
gtggagatgt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc 2760
acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga 2820
atgatagcct ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact 2880
gtcctttcga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt 2940
atcctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgacattt 3000
ttggagtaga ccagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg 3060
agtcgtaaaa gactctgtat gaactgttcg ccagtcttca cggcgagttc tgttagatcc 3120
tcgatttgaa tcttagactc catgcatggc cttagattca gtaggaacta cctttttaga 3180
gactccaatc tctattactt gccttggttt atgaagcaag ccttgaatcg tccatactgg 3240
aatagtactt ctgatcttga gaaatatgtc tttctctgtg ttcttgatgc aattagtcct 3300
gaatcttttg actgcatctt taaccttctt gggaaggtat ttgatctcct ggagattgtt 3360
actcgggtag atcgtcttga tgagacctgc tgcgtaggcc tctctaacca tctgtgggtc 3420
agcattcttt ctgaaattga agaggctaac cttctcatta tcagtggtga acatagtgtc 3480
gtcaccttca ccttcgaact tccttcctag atcgtaaaga tagaggaaat cgtccattgt 3540
aatctccggg gcaaaggaga tctcttttgg ggctggatca ctgctgggcc ttttggttcc 3600
tagcgtgagc cagtgggctt tttgctttgg tgggcttgtt agggccttag caaagctctt 3660
gggcttgagt tgagcttctc ctttggggat gaagttcaac ctgtctgttt gctgacttgt 3720
tgtgtacgcg tcagctgctg ctcttgcctc tgtaatagtg gcaaatttct tgtgtgcaac 3780
tccgggaacg ccgtttgttg ccgcctttgt acaaccccag tcatcgtata taccggcatg 3840
tggaccgtta tacacaacgt agtagttgat atgagggtgt tgaatacccg attctgctct 3900
gagaggagca actgtgctgt taagctcaga tttttgtggg attggaattg gatcgatctc 3960
gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat 4020
aattttgttt aactttaaga aggagatata cccatggaaa agcctgaact caccgcgacg 4080
tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 4140
gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 4200
gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 4260
gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 4320
tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 4380
gctgttctgc agccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag 4440
acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 4500
ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 4560
gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc 4620
gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc 4680
cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc 4740
gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc 4800
gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt 4860
ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg 4920
cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc 4980
gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga 5040
aaccgacgcc ccagcactcg tccgagggca aaggaatagt gaggtacagc ttggatcgat 5100
ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa 5160
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 5220
actatatccg gatgatcggg cgcgccgtcg acggatccgt acgagatccg gccggccaga 5280
tcctgcagga gatccaagct tttgatccat gcccttcatt tgccgcttat taattaattt 5340
ggtaacagtc cgtactaatc agttacttat ccttccccca tcataattaa tcttggtagt 5400
ctcgaatgcc acaacactga ctagtctctt ggatcataag aaaaagccaa ggaacaaaag 5460
aagacaaaac acaatgagag tatcctttgc atagcaatgt ctaagttcat aaaattcaaa 5520
caaaaacgca atcacacaca gtggacatca cttatccact agctgatcag gatcgccgcg 5580
tcaagaaaaa aaaactggac cccaaaagcc atgcacaaca acacgtactc acaaaggtgt 5640
caatcgagca gcccaaaaca ttcaccaact caacccatca tgagccctca catttgttgt 5700
ttctaaccca acctcaaact cgtattctct tccgccacct catttttgtt tatttcaaca 5760
cccgtcaaac tgcatgccac cccgtggcca aatgtccatg catgttaaca agacctatga 5820
ctataaatag ctgcaatctc ggcccaggtt ttcatcatca agaaccagtt caatatccta 5880
gtacaccgta ttaaagaatt taagatatac tgcggccgca ccatggaggt ggtgaatgaa 5940
atagtctcaa ttgggcagga agttttaccc aaagttgatt atgcccaact ctggagtgat 6000
gccagtcact gtgaggtgct ttacttgtcc atcgcatttg tcatcttgaa gttcactctt 6060
ggcccccttg gtccaaaagg tcagtctcgt atgaagtttg ttttcaccaa ttacaacctt 6120
ctcatgtcca tttattcgtt gggatcattc ctctcaatgg catatgccat gtacaccatc 6180
ggtgttatgt ctgacaactg cgagaaggct tttgacaaca acgtcttcag gatcaccacg 6240
cagttgttct atttgagcaa gttcctggag tatattgact ccttctattt gccactgatg 6300
ggcaagcctc tgacctggtt gcaattcttc catcatttgg gggcaccgat ggatatgtgg 6360
ctgttctata attaccgaaa tgaagctgtt tggatttttg tgctgttgaa tggtttcatc 6420
cactggatca tgtacggtta ttattggacc agattgatca agctgaagtt ccccatgcca 6480
aaatccctga ttacatcaat gcagatcatt caattcaatg ttggtttcta cattgtctgg 6540
aagtacagga acattccctg ttatcgccaa gatgggatga ggatgtttgg ctggttcttc 6600
aattactttt atgttggcac agtcttgtgt ttgttcttga atttctatgt gcaaacgtat 6660
atcgtcagga agcacaaggg agccaaaaag attcagtgag cggccgcaag tatgaactaa 6720
aatgcatgta ggtgtaagag ctcatggaga gcatggaata ttgtatccga ccatgtaaca 6780
gtataataac tgagctccat ctcacttctt ctatgaataa acaaaggatg ttatgatata 6840
ttaacactct atctatgcac cttattgttc tatgataaat ttcctcttat tattataaat 6900
catctgaatc gtgacggctt atggaatgct tcaaatagta caaaaacaaa tgtgtactat 6960
aagactttct aaacaattct aaccttagca ttgtgaacga gacataagtg ttaagaagac 7020
ataacaatta taatggaaga agtttgtctc catttatata ttatatatta cccacttatg 7080
tattatatta ggatgttaag gagacataac aattataaag agagaagttt gtatccattt 7140
atatattata tactacccat ttatatatta tacttatcca cttatttaat gtctttataa 7200
ggtttgatcc atgatatttc taatatttta gttgatatgt atatgaaagg gtactatttg 7260
aactctctta ctctgtataa aggttggatc atccttaaag tgggtctatt taattttatt 7320
gcttcttaca gataaaaaaa aaattatgag ttggtttgat aaaatattga aggatttaaa 7380
ataataataa ataacatata atatatgtat ataaatttat tataatataa catttatcta 7440
taaaaaagta aatattgtca taaatctata caatcgttta gccttgctgg acgaatctca 7500
attatttaaa cgagagtaaa catatttgac tttttggtta tttaacaaat tattatttaa 7560
cactatatga aatttttttt tttatcagca aagaataaaa ttaaattaag aaggacaatg 7620
gtgtcccaat ccttatacaa ccaacttcca caagaaagtc aagtcagaga caacaaaaaa 7680
acaagcaaag gaaatttttt aatttgagtt gtcttgtttg ctgcataatt tatgcagtaa 7740
aacactacac ataacccttt tagcagtaga gcaatggttg accgtgtgct tagcttcttt 7800
tattttattt ttttatcagc aaagaataaa taaaataaaa tgagacactt cagggatgtt 7860
tcaacaagct tgg 7873
//