Dbfetch
ID HC492080; SV 1; linear; unassigned DNA; PAT; SYN; 8185 BP. XX AC HC492080; XX DT 21-APR-2010 (Rel. 104, Created) DT 21-APR-2010 (Rel. 104, Last updated, Version 1) XX DE Sequence 11 from Patent WO2010031079. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RA Chotani G.K., Mcauliffe J.C., Peres C.M., Whited G.M.; RT "Systems using cell culture for production of isoprene"; RL Patent number WO2010031079-A1/11, 18-MAR-2010. RL The Goodyear Tire & Rubber Company (US); RL Danisco US Inc. (US). XX DR MD5; 14e25b00665af31ba885fbdee39c9662. DR SILVA-SSU; HC492080. XX FH Key Location/Qualifiers FH FT source 1..8185 FT /organism="synthetic construct" FT /mol_type="unassigned DNA" FT /note="Synthetic Construct" FT /db_xref="taxon:32630" XX SQ Sequence 8185 BP; 2057 A; 1916 C; 1829 G; 2383 T; 0 other; cgaccggtga gaagaacagc atcgggacaa gggaaggaag aacaaagaca aagaaaacaa 60 aagaaagcaa ttgaaaacaa aacaaaacaa ttttcattcc ttctcttatc attccttttc 120 ttttcttttc tctcattcaa cgcactccat cgtatccgta ttcctcttat tttttctctt 180 tctctatatc catttctttc tctctaggtg tgtcctctct ctctcttcaa tttctctact 240 ccgcattcca acgcatcctt cccccaacct cccatttcct ccttacggcc cgatagcgat 300 cgtctttccc tcgctatcac tcgctaccgg cccctcctct gcaccgtaac ctcctacgta 360 tttaccatat cataaagttt tttccgacgc ttatcgctga ccccctgtcg ccctcctatt 420 ggcttccgga ttatcttctt gtccataagg tgatccatgc ttcctgaaga ttcccgaaat 480 gtgtccactt tggcggggaa tcattccatc cacttctttc tctctcgctt tcctcattcg 540 gcgctcccct tccgcgtctc attggtcttc cgctccgttt ttgctttgcc gatgttactt 600 ggggagaggt gcgataatcc tttcgcaaaa actcggtttg acgcctccca tggtataaat 660 agtgggtggt ggacaggtgc cttcgctttt ctttaagcaa gagaatccca ttgtcttgac 720 tatcacgaat tcacatacat tatgaagatc accgctgtca ttgccctttt attctcactt 780 gctgctgcct cacctattcc agttgccgat cctggtgtgg tttcagttag caagtcatat 840 gctgatttcc ttcgtgttta ccaaagttgg aacacttttg ctaatcctga tagacccaac 900 cttaagaaga gaaatgatac acctgcaagt ggatatcaag ttgaaaaagt cgtaattttg 960 tcacgtcacg gtgttagggc ccctacaaaa atgactcaaa ccatgcgtga tgtcactcct 1020 aatacatggc cagaatggcc cgttaaatta ggatatatta caccaagagg tgaacacttg 1080 atatcactta tgggcggttt ttaccgtcaa aaattccagc aacaaggaat cctttctcag 1140 ggctcctgtc ctactcctaa ctccatatat gtctgggctg acgtcgatca gcgtacttta 1200 aaaactggtg aagcattcct tgctggtttg gcaccacaat gtggcttgac aattcatcac 1260 caacaaaatc ttgagaaagc tgatcctctt tttcatcccg ttaaagctgg aacctgctct 1320 atggataaaa ctcaagttca acaagctgtt gagaaggagg cacaaactcc tatagataat 1380 ttgaatcaac attacatccc ctttttagct ttaatgaata caacattaaa ttttagtact 1440 tctgcctggt gccaaaaaca ctctgctgat aaatcctgtg acctaggttt atccatgcct 1500 tctaaattgt ccataaaaga taatggtaac aaggtcgcat tggatggagc tattggtcta 1560 tcctctactt tggccgagat ttttcttctt gaatatgctc aaggcatgcc tcaagctgct 1620 tggggtaaca tccactcaga gcaagagtgg gcttccttgc taaagttgca taatgttcaa 1680 ttcgatttga tggcccgaac accttatatt gctcgacata acggtactcc tttattgcaa 1740 gctatatcaa atgcccttaa tcccaacgcc actgaatcaa aacttccaga tatttcacct 1800 gataacaaaa tattgttcat tgcaggtcat gacacaaata ttgctaatat agccggcatg 1860 ttaaatatgc gttggacatt accaggtcaa ccagataata ctcctccagg tggtgcccta 1920 gtatttgaac gtcttgctga taaaagtgga aaacaatatg tttctgtatc tatggtttat 1980 caaacactag aacaacttcg atcacagact cccctttctc taaatcagcc tgccggatct 2040 gttcaactta aaattccagg ttgcaatgat caaacagccg agggttactg tcctctttcc 2100 acttttacaa gagttgtttc ccaatctgtt gaacctggat gccaacttca ataatgagga 2160 tccaagtaag ggaatgagaa tgtgatccac ttttaattcc taatgaatac atgcctatag 2220 ttcttttctt ttgttcttta tgtcgttttt cgatggtacg gccgttgtca atctcagttt 2280 gtgtgcttgg ttgcagcttg gtttcaaatc tgttcatctc atgaatcttt taccatttca 2340 ccacacgttt ataccattct ctcatagaat cttcatcaaa ccatctcggg gttagagtgg 2400 aaagaaagtc ttgttctttt atttcctttt ttccatcttc aaggcttttc ttttcttcct 2460 cctcctcgtt catcttgagg tttgacgtgt ctgtttagaa ttttgagctg ttgcagcatc 2520 ttattttttg ttttgcgaaa acgaagcgct ttactctctt catcagttgg acgattgtac 2580 ctttgaaaac caactacttt tgcatgtttt gtatagaaat caatgatatt agaatcccat 2640 cctttaattt ctttcaaagt agttgagcta tagttaagtg taagggccct actgcgaaag 2700 catttgccaa ggatgttttc attaatcaag aacgaaagtt aggggatcga agacgatcag 2760 ataccgtcgt agtcttaacc ataaactatg ccgactaggg atcgggcaat gtttcattta 2820 tcgacttgct cggcacctta cgagaaatca aagtctttgg gttccggggg gagtatggtc 2880 gcaaggctga aacttaaagg aattgacgga agggcaccac aatggagtgg agcctgcggc 2940 ttaatttgac tcaacacggg gaaactcacc aggtccagac atagtaagga ttgacagatt 3000 gagagctctt tcttgattct atgggtggtg gtgcatggcc gttcttagtt ggtggagtga 3060 tttgtctgct taattgcgat aacgaacgag accttaacct gctaaatagc tggatcagcc 3120 attttggctg atcattagct tcttagaggg actattggca taaagccaat ggaagtttga 3180 ggcaataaca ggtctgtgat gcccttagat gttctgggcc gcacgcgcgc tacactgacg 3240 gagccaacga gttgaaaaaa atcttttgat tttttatcct tggccggaag gtctgggtaa 3300 tcttgttaaa ctccgtcgtg ctggggatag agcattgcaa ttattgcggc cgctcctcaa 3360 ttcgatgttg cagattttac aagtttttaa aatgtatttc attattactt tttatatgcc 3420 taataaaaaa gccatagttt aatctataga taactttttt tccagtgcac taacggacgt 3480 tacattccca tacaaaactg cgtagttaaa gctaaggaaa agttaatatc atgttaatta 3540 aatacgctat ttacaataag acattgaact catttatatc gttgaatatg aataaccaat 3600 ttcagcgaat ttttaacaaa catcgttcac ctcgtttaag gatatcttgt gtatggggtg 3660 ttgacttgct ttatcgaata attaccgtac ctgtaattgg cttgctggat atagcggtag 3720 tctaatatct agcaaaaatc ttttgggtga aaaggcttgc aatttcacga caccgaacta 3780 tttgtcattt tttaataagg aagttttcca taaattcctg taattctcgg ttgatctaat 3840 tgaaaagagt agttttgcat cacgatgagg agggcttttg tagaaagaaa tacgaacgaa 3900 acgaaaatca gcgttgccat cgctttggac aaagctccct tacctgaaga gtcgaatttt 3960 attgatgaac ttataacttc caagcatgca aaccaaaagg gagaacaagt aatccaagta 4020 gacacgggaa ttggattctt ggatcacatg tatcatgcac tggctaaaca tgcaggctgg 4080 agcttacgac tttactcaag aggtgattta atcatcgatg atcatcacac tgcagaagat 4140 actgctattg cacttggtat tgcattcaag caggctatgg gtaactttgc cggcgttaaa 4200 agatttggac atgcttattg tccacttgac gaagctcttt ctagaagcgt agttgacttg 4260 tcgggacggc cctatgctgt tatcgatttg ggattaaagc gtgaaaaggt tggggaattg 4320 tcctgtgaaa tgatccctca cttactatat tccttttcgg tagcagctgg aattactttg 4380 catgttacct gcttatatgg tagtaatgac catcatcgtg ctgaaagcgc ttttaaatct 4440 ctggctgttg ccatgcgcgc ggctactagt cttactggaa gttctgaagt cccaagcacg 4500 aagggagtgt tgtaaagatg aattggatta tgtcaggaaa agaacgacaa ttttgcatcc 4560 aaattgtcta aattttagag ttgcttgaaa acaatagaac cttacttgct ttataattac 4620 gttaattaga agcgttatct cgtgaaggaa tatagtacgt agccgtataa attgaattga 4680 atgttcagct tatagaatag agacactttg ctgttcaatg cgtcgtcact taccatactc 4740 actttattat acgactttaa gtataaactc cgcggttatg gtaaaattaa tgatgcacaa 4800 acgtccgatt ccatatgggt acactacaat taaatacttt taagctgatc ccccacacac 4860 catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc 4920 atcgccgtac cacttcaaaa cacccaagca cagcatacta aattttccct ctttcttcct 4980 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt 5040 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaat 5100 tttttttttt agtttttttc tctttcagtg acctccattg atatttaagt taataaacgg 5160 tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt tttttacttc 5220 ttgttcatta gaaagaaagc atagcaatct aatctaaggg cggtgttgac aattaatcat 5280 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt 5340 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga 5400 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg 5460 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg 5520 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca 5580 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc 5640 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg 5700 actgacacgt ccgacggcgg cccacgggtc ccaggcctcg gagatccgtc ccccttttcc 5760 tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc 5820 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 5880 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 5940 agacgcgagc ttcccagtaa atgtgccatc tcgtaggcag aaaacggttc ccccgtaggg 6000 tctctctctt ggcctccttt ctaggtcggg ctgattgctc ttgaagctct ctaggggggc 6060 tcacaccata ggcagataac gttccccacc ggctcgcctc gtaagcgcac aaggactgct 6120 cccaaagatc ctaggcggga ttttgccgat ttcggcctaa aggaaccgga acacgtagaa 6180 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac 6240 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 6300 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc 6360 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg 6420 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 6480 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 6540 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 6600 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 6660 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 6720 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6780 gtcatctcgc cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6840 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6900 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggaca agagcatcag 6960 gggctcgcgc cagccgaact gttcgccagg ctcaaggccg catgcccgac ggcgaggatc 7020 tcgtcgtgat ccatggcgat gcctgctgcc gaatatcatg gtggaaaatg gccgcttttc 7080 tggattaacg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgtggata 7140 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 7200 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 7260 gaattgaaaa aggtaccaag tttactcata tatactttag attgatttaa aacttcattt 7320 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 7380 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 7440 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 7500 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 7560 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 7620 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 7680 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 7740 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 7800 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag 7860 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 7920 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 7980 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 8040 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 8100 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 8160 cagccgaacg accgagcgca gcgag 8185 //