Sequence Similarity Search

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Current parameters

Sequence set: Assembled and annotated sequences

Program: blastn

Task: megablast

Max scores: 50

Max alignments: 50

Threshold: 10

Alignment view: pairwise

Filter low complexity regions: true

Match/Mismatch scores: 2,-3

Gap costs: Linear

Drop off: 0

Align using gaps: false

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 Assembled and annotated sequences

 Barcode sequences

 Coding sequences

 Geo-referenced sequences

 Non-coding sequences

 Vectors (Emvec)

 Limit sequence by:

 Taxonomic group

 Data class

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Program

blastntblastxtblastn

 megablast (recommended for highly similar sequences)

 blastn (recommended for less similar sequences)

More options

Result options

Maximum scores:

51020501001502002505007501000

Maximum alignments:

51020501001502002505007501000

Expect threshold:

1e-2001e-1001e-501e-101e-51e-41e-31e-21e-11.0101001000

Alignment view:

pairwiseQuery-anchored identitiesQuery-anchored non-identitiesFlat query-anchored identitiesFlat query-anchored non-identitiesBLASTXML

 Filter low complexity regions

Scoring options

Match/mismatch scores:

2,-71,-32,-51,-22,-31,-15,-44,-5

Gap costs:

LinearExistence: 5 Extension: 5Existence: 4 Extension: 4Existence: 2 Extension: 4Existence: 0 Extension: 4Existence: 3 Extension: 3Existence: 6 Extension: 2Existence: 5 Extension: 2Existence: 4 Extension: 2Existence: 2 Extension: 2Existence: 6 Extension: 4Existence: -1 Extension: -1Existence: 5 Extension: -1Existence: -1 Extension: 5Existence: -1 Extension: 2

Drop off:

0246810

General options

 Align using gaps

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Current parameters

Sequence set: Assembled and annotated sequences

Program: blastn

Task: megablast

Max scores: 50

Max alignments: 50

Threshold: 10

Alignment view: pairwise

Filter low complexity regions: true

Match/Mismatch scores: 2,-3

Gap costs: Linear

Drop off: 0

Align using gaps: false

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