S. lacrymans monokaryons S7.9 and S7.3 were prepared from the S. lacrymans S7 dikaryon. The strain S7.3 was sequenced with 454 (Roche) pyrosequencing and assembled using assembled S7.9 (sequenced using Sanger platform) as a template for scaffolding. Interestingly the S7.3 genome contained more genes than the S7.9 genome.

Comment
URL -- http://www.jgi.doe.gov/Serpula~JGI Project ID: 4083953~The DNA was provided by Daniel Eastwood (d.c.estwood@swansea.ac.uk)~The parental strain of Serpula lacrymans was isolated and identified by J.Liese from a building in Berlin in 1937. Fruiting body and monokaryons 7.3 and 7.9 were produced by O. Schmidt and U. Moreth, Hamburg, in 1991. Strains are stored in culture collection University Hamburg (O. Schmidt).~Contacts: Daniel Eastwood (d.c.eastwood@swansea.ac.uk)~ Igor Grigoriev (ivgrigoriev@lbl.gov)~Assembly and annotation done by JGI.