H
IPR042082

CST complex subunit Stn1, wHTH1 motif superfamily

InterPro entry
Short nameCST_Stn1_wHTH1_sf
Overlapping entries
 
Stn1, C-terminal (IPR015253)

Description

Stn1 is a component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites
[3, 2, 1]
.

The C-terminal domain of Stn1 has two winged helix-turn-helix (wHTH) motifs, wHTH1 and wHTH2. This superfamily represents the wHTH1 motif, which is structurally similar to that in RPA32 with an additional large insertion between helices α2 and α3, unique to Stn1
[4, 5]
. This additional wHTH1 motif may allow interaction with a different set of proteins that function at telomeres such as Ctc1
[5]
.

References

1.Human CST abundance determines recovery from diverse forms of DNA damage and replication stress. Wang F, Stewart J, Price CM. Cell Cycle 13, 3488-98, (2014). View articlePMID: 25483097

2.OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation. Wan M, Qin J, Songyang Z, Liu D. J. Biol. Chem. 284, 26725-31, (2009). View articlePMID: 19648609

3.RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway. Miyake Y, Nakamura M, Nabetani A, Shimamura S, Tamura M, Yonehara S, Saito M, Ishikawa F. Mol. Cell 36, 193-206, (2009). View articlePMID: 19854130

4.Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres. Sun J, Yu EY, Yang Y, Confer LA, Sun SH, Wan K, Lue NF, Lei M. Genes Dev. 23, 2900-14, (2009). View articlePMID: 20008938

5.Structure of the human telomeric Stn1-Ten1 capping complex. Bryan C, Rice C, Harkisheimer M, Schultz DC, Skordalakes E. PLoS One 8, e66756, (2013). PMID: 23826127

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