› 1ng2

X-ray diffraction
1.7Å resolution

Structure of autoinhibited p47phox

Released:
DOI: 10.2210/pdb1ng2/pdb
PDB entry 1ng2 coloured by chain, front view.
PDB entry 1ng2 coloured by chain, side view.
PDB entry 1ng2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of phosphorylation-induced activation of the NADPH oxidase.
Groemping Y, Lapouge K, Smerdon SJ, Rittinger K
Cell 113 343-55 (2003)
PMID: 12732142

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-146972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil cytosol factor 1 Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14598 (Residues: 156-340; Coverage: 47%)
Gene names: NCF1, NOXO2, SH3PXD1A
Sequence domains:
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P41212
Unit cell:
a: 99.986Å b: 99.986Å c: 44.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

73 review citations

The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.
Bedard et al. (2007)
72 more

12 mentions without citation

Activation and assembly of the NADPH oxidase: a structural perspective.
Groemping et al. (2005)
11 more