› 1a2n

X-ray diffraction
2.8Å resolution

STRUCTURE OF THE C115A MUTANT OF MURA COMPLEXED WITH THE FLUORINATED ANALOG OF THE REACTION TETRAHEDRAL INTERMEDIATE

Released:
DOI: 10.2210/pdb1a2n/pdb
PDB entry 1a2n coloured by chain, front view.
PDB entry 1a2n coloured by chain, side view.
PDB entry 1a2n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA.
Skarzynski T, Kim DH, Lees WJ, Walsh CT, Duncan K
Biochemistry 37 2572-7 (1998)
PMID: 9485407

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 44.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A749 (Residues: 1-419; Coverage: 100%)
Gene names: JW3156, b3189, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

1 bound ligand:
Ligand TET 1 x TET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P321
Unit cell:
a: 111.15Å b: 111.15Å c: 67.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 not available
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
11 more

7 mentions without citation

Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail.
Schönbrunn et al. (2001)
6 more