› 1a2o

X-ray diffraction
2.4Å resolution

STRUCTURAL BASIS FOR METHYLESTERASE CHEB REGULATION BY A PHOSPHORYLATION-ACTIVATED DOMAIN

Released:
DOI: 10.2210/pdb1a2o/pdb
PDB entry 1a2o coloured by chain, front view.
PDB entry 1a2o coloured by chain, side view.
PDB entry 1a2o coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain.
Djordjevic S, Goudreau PN, Xu Q, Stock AM, West AH
Proc Natl Acad Sci U S A 95 1381-6 (1998)
PMID: 9465023

Function and Biology Details

Reactions catalysed: 
Protein L-glutamate O(5)-methyl ester + H(2)O = protein L-glutamate + methanol
Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-glutamate methylesterase/protein-glutamine glutaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 37.6 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P04042 (Residues: 1-349; Coverage: 100%)
Gene names: STM1917, cheB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 163.83Å b: 100.46Å c: 53.12Å
α: 90° β: 98.63° γ: 90°
R-values:
R R work R free
0.225 0.222 not available
Expression system: Escherichia coli

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Citations

24 review citations

Two-component signal transduction.
Stock et al. (2000)
23 more

16 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
15 more