› 1a54

X-ray diffraction
1.6Å resolution

PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION

Released:
DOI: 10.2210/pdb1a54/pdb
PDB entry 1a54 coloured by chain, front view.
PDB entry 1a54 coloured by chain, side view.
PDB entry 1a54 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate.
Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR
Biochemistry 37 10381-5 (1998)
PMID: 9671506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG82 (Residues: 26-346; Coverage: 100%)
Gene names: JW3706, b3728, phoS, pstS
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand 2HP 1 x 2HP
Ligand MDC 1 x MDC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P212121
Unit cell:
a: 41.498Å b: 62.651Å c: 122.608Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.208
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
8 more