› 1a5k

X-ray diffraction
2.2Å resolution

K217E VARIANT OF KLEBSIELLA AEROGENES UREASE

Released:
DOI: 10.2210/pdb1a5k/pdb
PDB entry 1a5k coloured by chain, front view.
PDB entry 1a5k coloured by chain, side view.
PDB entry 1a5k coloured by chain, top view.
Source organism: Klebsiella aerogenes
Primary publication:
Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand.
Pearson MA, Schaller RA, Michel LO, Karplus PA, Hausinger RP
Biochemistry 37 6214-20 (1998)
PMID: 9558361

Function and Biology Details

Reaction catalysed: 
Urea + H(2)O = CO(2) + 2 NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-148349 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.1 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18316 (Residues: 1-100; Coverage: 100%)
Gene name: ureA
Sequence domains: Urease, gamma subunit
Structure domains: Urease, gamma-like subunit
Urease subunit beta Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.13 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18315 (Residues: 1-101; Coverage: 95%)
Gene name: ureB
Sequence domains: Urease beta subunit
Structure domains: Urease, beta subunit
Urease subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 566 amino acids
Theoretical weight: 60.24 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18314 (Residues: 2-567; Coverage: 100%)
Gene name: ureC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I213
Unit cell:
a: 170.8Å b: 170.8Å c: 170.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Nickel uptake and utilization by microorganisms.
Mulrooney et al. (2003)
6 more

2 mentions without citation

Nonredox nickel enzymes.
Maroney et al. (2014)
1 more