› 1a6j

X-ray diffraction
2.35Å resolution

NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN

Released:
DOI: 10.2210/pdb1a6j/pdb
PDB entry 1a6j coloured by chain, front view.
PDB entry 1a6j coloured by chain, side view.
PDB entry 1a6j coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli.
Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH, Dijkstra BW
J Mol Biol 279 245-55 (1998)
PMID: 9636714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 17.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69829 (Residues: 1-163; Coverage: 100%)
Gene names: JW3171, b3204, ptsN, rpoP, yhbI
Sequence domains: Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

2 bound ligands:
Ligand BME 2 x BME
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P43212
Unit cell:
a: 107.9Å b: 107.9Å c: 64.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.251
Expression system: Escherichia coli

Quick links

Citations

6 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
5 more

6 mentions without citation

Mechanochemistry in Translation.
Leininger et al. (2019)
5 more