› 1apm

X-ray diffraction
2Å resolution

2.0 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH A PEPTIDE INHIBITOR AND DETERGENT

Released:
DOI: 10.2210/pdb1apm/pdb
PDB entry 1apm coloured by chain, front view.
PDB entry 1apm coloured by chain, side view.
PDB entry 1apm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
2.0 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with a peptide inhibitor and detergent.
Knighton DR, Bell SM, Zheng J, Ten Eyck LF, Xuong NH, Taylor SS, Sowadski JM
Acta Crystallogr D Biol Crystallogr 49 357-61 (1993)
PMID: 15299526

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.72 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P63248 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments

1 bound ligand:
Ligand OCT 1 x OCT
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 73.84Å b: 75.76Å c: 81.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Not provided

Quick links

Citations

7 review citations

Mechanisms of specificity in protein phosphorylation.
Ubersax et al. (2007)
6 more

14 mentions without citation

Solution NMR Spectroscopy for the Study of Enzyme Allostery.
Lisi et al. (2016)
13 more