› 1bf6

X-ray diffraction
1.7Å resolution

PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1bf6/pdb
PDB entry 1bf6 coloured by chain, front view.
PDB entry 1bf6 coloured by chain, side view.
PDB entry 1bf6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Biochemical characterization and crystallographic structure of an Escherichia coli protein from the phosphotriesterase gene family.
Buchbinder JL, Stephenson RC, Dresser MJ, Pitera JW, Scanlan TS, Fletterick RJ
Biochemistry 37 5096-106 (1998)
PMID: 9548740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphotriesterase homology protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 32.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P45548 (Residues: 2-292; Coverage: 100%)
Gene names: JW3342, b3379, php, yhfV
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 2 x SO4
Ligand MPD 1 x MPD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 42.07Å b: 80.8Å c: 98.2Å
α: 90° β: 97.1° γ: 90°
R-values:
R R work R free
0.204 0.204 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
1 more

12 mentions without citation

Functional annotation and three-dimensional structure of Dr0930 from Deinococcus radiodurans, a close relative of phosphotriesterase in the amidohydrolase superfamily.
Xiang et al. (2009)
11 more