› 1bq7

X-ray diffraction
2.8Å resolution

DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA

Released:
DOI: 10.2210/pdb1bq7/pdb
PDB entry 1bq7 coloured by chain, front view.
PDB entry 1bq7 coloured by chain, side view.
PDB entry 1bq7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
On the role of the cis-proline residue in the active site of DsbA.
Charbonnier JB, Belin P, Moutiez M, Stura EA, Quéméneur E
Protein Sci 8 96-105 (1999)
PMID: 10210188

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 189 amino acids
Theoretical weight: 21.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG4 (Residues: 20-208; Coverage: 100%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P21
Unit cell:
a: 64.9Å b: 190.7Å c: 67.1Å
α: 90° β: 111.9° γ: 90°
R-values:
R R work R free
0.218 0.218 0.289
Expression system: Escherichia coli

Quick links

Citations

5 review citations

How proteins form disulfide bonds.
Depuydt et al. (2011)
4 more