› 1bt7

Solution NMR

THE SOLUTION NMR STRUCTURE OF THE N-TERMINAL PROTEASE DOMAIN OF THE HEPATITIS C VIRUS (HCV) NS3-PROTEIN, FROM BK STRAIN, 20 STRUCTURES

Released:
DOI: 10.2210/pdb1bt7/pdb
PDB entry 1bt7 coloured by chain, ensemble of 20 models, front view.
PDB entry 1bt7 coloured by chain, ensemble of 20 models, side view.
PDB entry 1bt7 coloured by chain, ensemble of 20 models, top view.
Source organism: Hepacivirus hominis
Primary publication:
The solution structure of the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism.
Barbato G, Cicero DO, Nardi MC, Steinkühler C, Cortese R, De Francesco R, Bazzo R
J Mol Biol 289 371-84 (1999)
PMID: 10366511

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease/helicase NS3 Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 19.63 KDa
Source organism: Hepacivirus hominis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26663 (Residues: 1027-1207; Coverage: 6%)
Sequence domains: Hepatitis C virus NS3 protease
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DG-SA HYBRID
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Recent advances in the molecular biology of hepatitis C virus.
Rosenberg S. (2001)
9 more

4 mentions without citation

NS3 protease from hepatitis C virus: biophysical studies on an intrinsically disordered protein domain.
Vega et al. (2013)
3 more