1c5k

X-ray diffraction
2Å resolution

THE STRUCTURE OF TOLB, AN ESSENTIAL COMPONENT OF THE TOL-DEPENDENT TRANSLOCATION SYSTEM AND ITS INTERACTIONS WITH THE TRANSLOCATION DOMAIN OF COLICIN E9

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-141664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tol-Pal system protein TolB Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 47.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A855 (Residues: 1-430; Coverage: 100%)
Gene names: JW5100, b0740, tolB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21
Unit cell:
a: 63.58Å b: 40.19Å c: 77.66Å
α: 90° β: 110.22° γ: 90°
R-values:
R R work R free
0.234 0.234 0.271
Expression system: Escherichia coli BL21(DE3)