› 1ci1

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM TRYPANOSOMA CRUZI IN HEXANE

Released:
DOI: 10.2210/pdb1ci1/pdb
PDB entry 1ci1 coloured by chain, front view.
PDB entry 1ci1 coloured by chain, side view.
PDB entry 1ci1 coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Crystal structure of triosephosphate isomerase from Trypanosoma cruzi in hexane.
Gao XG, Maldonado E, Pérez-Montfort R, Garza-Ramos G, de Gómez-Puyou MT, Gómez-Puyou A, Rodríguez-Romero A
Proc Natl Acad Sci U S A 96 10062-7 (1999)
PMID: 10468562

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 27.36 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: P52270 (Residues: 1-251; Coverage: 100%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand HEX 3 x HEX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 43.656Å b: 77.65Å c: 149.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.239
Expression system: Escherichia coli

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Citations

2 review citations

Improving enzymes by using them in organic solvents.
Klibanov AM. (2001)
1 more

4 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
3 more