› 1cja

X-ray diffraction
2.9Å resolution

ACTIN-FRAGMIN KINASE, CATALYTIC DOMAIN FROM PHYSARUM POLYCEPHALUM

Released:
DOI: 10.2210/pdb1cja/pdb
PDB entry 1cja coloured by chain, front view.
PDB entry 1cja coloured by chain, side view.
PDB entry 1cja coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domain.
Steinbacher S, Hof P, Eichinger L, Schleicher M, Gettemans J, Vandekerckhove J, Huber R, Benz J
EMBO J 18 2923-9 (1999)
PMID: 10357805

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-fragmin kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 38.1 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: P80197 (Residues: 2-343; Coverage: 46%)
Sequence domains: Actin-fragmin kinase, catalytic
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 178.9Å b: 178.9Å c: 59.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural insights into actin-binding, branching and bundling proteins.
Winder SJ. (2003)
2 more

6 mentions without citation

Design principles underpinning the regulatory diversity of protein kinases.
Oruganty et al. (2012)
5 more