› 1cko

X-ray diffraction
3.1Å resolution

STRUCTURE OF MRNA CAPPING ENZYME IN COMPLEX WITH THE CAP ANALOG GPPPG

Released:
DOI: 10.2210/pdb1cko/pdb
PDB entry 1cko coloured by chain, front view.
PDB entry 1cko coloured by chain, side view.
PDB entry 1cko coloured by chain, top view.
Source organism: Paramecium bursaria Chlorella virus 1
Primary publication:
Structure of a complex between a cap analogue and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping.
Hâkansson K, Wigley DB
Proc Natl Acad Sci U S A 95 1505-10 (1998)
PMID: 9465045

Function and Biology Details

Reaction catalysed: 
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 37.88 KDa
Source organism: Paramecium bursaria Chlorella virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84424 (Residues: 1-330; Coverage: 100%)
Gene name: A103R
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GP3 1 x GP3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: C2221
Unit cell:
a: 78.476Å b: 164.013Å c: 103.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.314
Expression system: Escherichia coli

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Citations

7 review citations

Eukaryotic DNA ligases: structural and functional insights.
Ellenberger et al. (2008)
6 more

5 mentions without citation

Conventional and unconventional mechanisms for capping viral mRNA.
Decroly et al. (2011)
4 more