› 1cku

X-ray diffraction
1.2Å resolution

AB INITIO SOLUTION AND REFINEMENT OF TWO HIGH POTENTIAL IRON PROTEIN STRUCTURES AT ATOMIC RESOLUTION

Released:
DOI: 10.2210/pdb1cku/pdb
PDB entry 1cku coloured by chain, front view.
PDB entry 1cku coloured by chain, side view.
PDB entry 1cku coloured by chain, top view.
Source organism: Allochromatium vinosum
Primary publication:
Ab initio solution and refinement of two high-potential iron protein structures at atomic resolution.
Parisini E, Capozzi F, Lubini P, Lamzin V, Luchinat C, Sheldrick GM
Acta Crystallogr D Biol Crystallogr 55 1773-84 (1999)
PMID: 10531472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High-potential iron-sulfur protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 8.91 KDa
Source organism: Allochromatium vinosum
Expression system: Escherichia coli
UniProt:
  • Canonical: P00260 (Residues: 38-122; Coverage: 100%)
Gene names: Alvin_2274, hip
Sequence domains: High potential iron-sulfur protein
Structure domains: High potential iron-sulphur protein

Ligands and Environments

1 bound ligand:
Ligand SF4 2 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 35.067Å b: 51.389Å c: 92.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 not available 0.178
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more

11 mentions without citation

Protein unfolding: rigidity lost.
Rader et al. (2002)
10 more