› 1czw

X-ray diffraction
2.5Å resolution

STRUCTURE OF THE W34A MUTANT OF SHIGA-LIKE TOXIN I B SUBUNIT

Released:
DOI: 10.2210/pdb1czw/pdb
PDB entry 1czw coloured by chain, front view.
PDB entry 1czw coloured by chain, side view.
PDB entry 1czw coloured by chain, top view.
Source organism: Phage h30
Entry authors: Ling H, Boodhoo A, Brunton JL, Read RJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shiga-like toxin 1 subunit B Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 69 amino acids
Theoretical weight: 7.58 KDa
Source organism: Phage h30
Expression system: Escherichia coli
UniProt:
  • Canonical: P69179 (Residues: 21-89; Coverage: 100%)
Gene names: sltB, stxB
Sequence domains: Shiga-like toxin beta subunit
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21212
Unit cell:
a: 107.55Å b: 108.92Å c: 57.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.214
Expression system: Escherichia coli

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