› 1d1k

X-ray diffraction
2Å resolution

MUTATED SHIGA-LIKE TOXIN B SUBUNIT (D17E/W34A) COMPLEXED WITH RECEPTOR GB3 ANALOGUE

Released:
DOI: 10.2210/pdb1d1k/pdb
PDB entry 1d1k coloured by chain, front view.
PDB entry 1d1k coloured by chain, side view.
PDB entry 1d1k coloured by chain, top view.
Source organism: Phage h30
Entry authors: Ling H, Brunton JL, Read RJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Shiga-like toxin 1 subunit B Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 69 amino acids
Theoretical weight: 7.6 KDa
Source organism: Phage h30
Expression system: Escherichia coli
UniProt:
  • Canonical: P69178 (Residues: 21-89; Coverage: 100%)
Gene name: stxB
Sequence domains: Shiga-like toxin beta subunit
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL, GLA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P1
Unit cell:
a: 44.223Å b: 44.131Å c: 53.71Å
α: 106.05° β: 106.38° γ: 99.26°
R-values:
R R work R free
0.193 0.193 0.218
Expression system: Escherichia coli

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