› 1d3n

X-ray diffraction
2Å resolution

METHIONINE CORE MUTATION

Released:
DOI: 10.2210/pdb1d3n/pdb
PDB entry 1d3n coloured by chain, front view.
PDB entry 1d3n coloured by chain, side view.
PDB entry 1d3n coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Use of differentially substituted selenomethionine proteins in X-ray structure determination.
Gassner NC, Matthews BW
Acta Crystallogr D Biol Crystallogr 55 1967-70 (1999)
PMID: 10666571

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 19.19 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand HED 1 x HED
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 61.26Å b: 61.26Å c: 96.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.167 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Advances in multiple wavelength anomalous diffraction crystallography.
Ealick SE. (2000)
13 other articles

2 mentions without citation

TSpred: a web server for the rational design of temperature-sensitive mutants.
Tan et al. (2014)
1 more