› 1dcm

X-ray diffraction
3Å resolution

STRUCTURE OF UNPHOSPHORYLATED FIXJ-N WITH AN ATYPICAL CONFORMER (MONOMER A)

Released:
DOI: 10.2210/pdb1dcm/pdb
PDB entry 1dcm coloured by chain, front view.
PDB entry 1dcm coloured by chain, side view.
PDB entry 1dcm coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Primary publication:
Structural transitions in the FixJ receiver domain.
Gouet P, Fabry B, Guillet V, Birck C, Mourey L, Kahn D, Samama JP
Structure 7 1517-26 (1999)
PMID: 10647182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein FixJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.88 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli
UniProt:
  • Canonical: P10958 (Residues: 1-126; Coverage: 62%)
Gene names: RA0669, SMa1227, fixJ
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 115.6Å b: 34.8Å c: 66.9Å
α: 90° β: 119.4° γ: 90°
R-values:
R R work R free
0.258 0.248 0.335
Expression system: Escherichia coli

Quick links

Citations

27 citation in other articles

Conformational changes in protein loops and helices induced by post-translational phosphorylation.
Groban et al. (2006)
26 more

1 mention without citation

Automated refinement of macromolecular structures at low resolution using prior information.
Kovalevskiy et al. (2016)