› 1dm3

X-ray diffraction
2Å resolution

ACETYLATED BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH ACETYL-COA

Released:
DOI: 10.2210/pdb1dm3/pdb
PDB entry 1dm3 coloured by chain, front view.
PDB entry 1dm3 coloured by chain, side view.
PDB entry 1dm3 coloured by chain, top view.
Source organism: Zoogloea ramigera
Primary publication:
Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase.
Modis Y, Wierenga RK
J Mol Biol 297 1171-82 (2000)
PMID: 10764581

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 40.3 KDa
Source organism: Zoogloea ramigera
Expression system: Escherichia coli
UniProt:
  • Canonical: P07097 (Residues: 5-10, 11-392; Coverage: 99%)
Gene names: phaA, phbA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand ACO 4 x ACO
1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand SCY 4 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21
Unit cell:
a: 84.384Å b: 78.846Å c: 149.729Å
α: 90° β: 93.51° γ: 90°
R-values:
R R work R free
0.214 0.214 0.256
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
6 more

14 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
13 more