› 1doq

Solution NMR

THE C-TERMINAL DOMAIN OF THE RNA POLYMERASE ALPHA SUBUNIT FROM THERMUS THERMOPHILUS

Released:
DOI: 10.2210/pdb1doq/pdb
PDB entry 1doq coloured by chain, front view.
PDB entry 1doq coloured by chain, side view.
PDB entry 1doq coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
The structure and the characteristic DNA binding property of the C-terminal domain of the RNA polymerase alpha subunit from Thermus thermophilus.
Wada T, Yamazaki T, Kyogoku Y
J Biol Chem 275 16057-63 (2000)
PMID: 10821859

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.79 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z9H6 (Residues: 247-315; Coverage: 22%)
Gene name: rpoA
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY, SIMULATED ANNEALING, MOLECULAR DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4.
Ross et al. (2003)
7 more

4 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
3 more