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X-ray diffraction
2.8Å resolution

STRUCTURE OF LARGE FRAGMENT OF ESCHERICHIA COLI DNA POLYMERASE I COMPLEXED WITH D/TMP

Released:
DOI: 10.2210/pdb1dpi/pdb
PDB entry 1dpi coloured by chain, front view.
PDB entry 1dpi coloured by chain, side view.
PDB entry 1dpi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA
Nature 313 762-6
PMID: 3883192

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.16 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P00582 (Residues: 324-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43
Unit cell:
a: 102.9Å b: 102.9Å c: 85.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 not available not available
Expression system: Not provided

Quick links

Citations

94 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
93 more

5 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
4 more