› 1dts

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF AN ATP DEPENDENT CARBOXYLASE, DETHIOBIOTIN SYNTHASE, AT 1.65 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1dts/pdb
PDB entry 1dts coloured by chain, front view.
PDB entry 1dts coloured by chain, side view.
PDB entry 1dts coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of an ATP-dependent carboxylase, dethiobiotin synthetase, at 1.65 A resolution.
Huang W, Lindqvist Y, Schneider G, Gibson KJ, Flint D, Lorimer G
Structure 2 407-14 (1994)
PMID: 8081756

Function and Biology Details

Reaction catalysed: 
ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent dethiobiotin synthetase BioD 1 Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 23.71 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P13000 (Residues: 1-225; Coverage: 98%)
Gene names: JW0761, b0778, bioD1
Sequence domains: CobQ/CobB/MinD/ParA nucleotide binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 73.2Å b: 49.2Å c: 61.8Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.169 0.169 0.23
Expression system: Not provided

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Citations

4 review citations

Biotin in microbes, the genes involved in its biosynthesis, its biochemical role and perspectives for biotechnological production.
Streit et al. (2003)
3 more

3 mentions without citation

Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
Dey et al. (2010)
2 more