› 1dvi

X-ray diffraction
2.3Å resolution

CALPAIN DOMAIN VI WITH CALCIUM BOUND

Released:
DOI: 10.2210/pdb1dvi/pdb
PDB entry 1dvi coloured by chain, front view.
PDB entry 1dvi coloured by chain, side view.
PDB entry 1dvi coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of a calpain Ca(2+)-binding domain reveals a novel EF-hand and Ca(2+)-induced conformational changes.
Blanchard H, Grochulski P, Li Y, Arthur JS, Davies PL, Elce JS, Cygler M
Nat Struct Biol 4 532-8 (1997)
PMID: 9228945

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calpain small subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64537 (Residues: 88-270; Coverage: 68%)
Gene names: Capn4, Capns1, Css1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 75Å b: 87.4Å c: 118.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

The calpain system.
Goll et al. (2003)
25 more

9 mentions without citation

Crystal structure of calcium-free human sorcin: a member of the penta-EF-hand protein family.
Xie et al. (2001)
8 more