› 1e1n

X-ray diffraction
2.4Å resolution

Structure of adrenodoxin reductase at 2.4 Angstrom in crystal form A'

Released:
DOI: 10.2210/pdb1e1n/pdb
PDB entry 1e1n coloured by chain, front view.
PDB entry 1e1n coloured by chain, side view.
PDB entry 1e1n coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
Ziegler GA, Schulz GE
Biochemistry 39 10986-95 (2000)
PMID: 10998235

Function and Biology Details

Reaction catalysed: 
2 reduced adrenodoxin + NADP(+) = 2 oxidized adrenodoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH:adrenodoxin oxidoreductase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 50.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08165 (Residues: 33-492; Coverage: 94%)
Gene names: ADXR, FDXR
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 57.8Å b: 62Å c: 83Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.201 0.201 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Open questions in ferredoxin-NADP+ reductase catalytic mechanism.
Carrillo et al. (2003)
8 more

3 mentions without citation

Protein-protein docking benchmark version 3.0.
Hwang et al. (2008)
2 more