› 1e2a

X-ray diffraction
2.3Å resolution

ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS

Released:
DOI: 10.2210/pdb1e2a/pdb
PDB entry 1e2a coloured by chain, front view.
PDB entry 1e2a coloured by chain, side view.
PDB entry 1e2a coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The structure of enzyme IIAlactose from Lactococcus lactis reveals a new fold and points to possible interactions of a multicomponent system.
Sliz P, Engelmann R, Hengstenberg W, Pai EF
Structure 5 775-88 (1997)
PMID: 9261069

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-150002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system lactose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.46 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P23532 (Residues: 1-105; Coverage: 100%)
Gene name: lacF
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P41212
Unit cell:
a: 90.9Å b: 90.9Å c: 82.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.24
Expression system: Escherichia coli

Quick links

Citations

7 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
6 more

8 mentions without citation

Prediction of functional sites by analysis of sequence and structure conservation.
Panchenko et al. (2004)
7 more