› 1e2v

X-ray diffraction
1.85Å resolution

N153Q mutant of cytochrome f from Chlamydomonas reinhardtii

Released:
DOI: 10.2210/pdb1e2v/pdb
PDB entry 1e2v coloured by chain, front view.
PDB entry 1e2v coloured by chain, side view.
PDB entry 1e2v coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Interruption of the internal water chain of cytochrome f impairs photosynthetic function.
Sainz G, Carrell CJ, Ponamarev MV, Soriano GM, Cramer WA, Smith JL
Biochemistry 39 9164-73 (2000)
PMID: 10924110

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome f Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 251 amino acids
Theoretical weight: 27.2 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P23577 (Residues: 32-282; Coverage: 88%)
Gene name: petA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HEC 3 x HEC
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 76.12Å b: 94.22Å c: 121.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.219
Expression system: Escherichia coli

Quick links

Citations

11 review citations

pH-dependent regulation of electron transport and ATP synthesis in chloroplasts.
Tikhonov AN. (2013)
10 more