› 1e8s

X-ray diffraction
4Å resolution

Alu domain of the mammalian SRP (potential Alu retroposition intermediate)

Released:
DOI: 10.2210/pdb1e8s/pdb
PDB entry 1e8s coloured by chain, front view.
PDB entry 1e8s coloured by chain, side view.
PDB entry 1e8s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and assembly of the Alu domain of the mammalian signal recognition particle.
Weichenrieder O, Wild K, Strub K, Cusack S
Nature 408 167-73 (2000)
PMID: 11089964

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153354 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 9 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 10 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49458 (Residues: 2-86; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Signal recognition particle 14 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 12.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37108 (Residues: 2-107; Coverage: 78%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
7SL RNA, 88-MER Chain: C
Molecule details ›
Chain: C
Length: 88 nucleotides
Theoretical weight: 28.49 KDa

Ligands and Environments

1 bound ligand:
Ligand EU3 2 x EU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P42212
Unit cell:
a: 143.33Å b: 143.33Å c: 60.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.388 0.388 not available
Expression system: Escherichia coli

Quick links

Citations

29 review citations

The signal recognition particle.
Keenan et al. (2001)
28 more

5 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
4 more