1ee9

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH NAD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-169660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate dehydrogenase [NAD(+)] Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 36.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02046 (Residues: 1-320; Coverage: 100%)
Gene names: MTD1, YKR080W, YKR400
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 73.6Å b: 73.6Å c: 161.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.248 0.36
Expression system: Escherichia coli