› 1eke

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF CLASS II RIBONUCLEASE H (RNASE HII) WITH MES LIGAND

Released:
DOI: 10.2210/pdb1eke/pdb
PDB entry 1eke coloured by chain, front view.
PDB entry 1eke coloured by chain, side view.
PDB entry 1eke coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of archaeal RNase HII: a homologue of human major RNase H.
Lai L, Yokota H, Hung LW, Kim R, Kim SH
Structure 8 897-904 (2000)
PMID: 10997908

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.69 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57599 (Residues: 1-230; Coverage: 100%)
Gene names: MJ0135, rnhB
Sequence domains: Ribonuclease HII
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MES 2 x MES
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 61.484Å b: 55.424Å c: 72.145Å
α: 90° β: 111.85° γ: 90°
R-values:
R R work R free
0.213 0.21 0.262
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Retroviral integrase superfamily: the structural perspective.
Nowotny M. (2009)
8 more

4 mentions without citation

The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification.
Majorek et al. (2014)
3 more