› 1eud

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF PHOSPHORYLATED PIG HEART, GTP-SPECIFIC SUCCINYL-COA SYNTHETASE

Released:
DOI: 10.2210/pdb1eud/pdb
PDB entry 1eud coloured by chain, front view.
PDB entry 1eud coloured by chain, side view.
PDB entry 1eud coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Phosphorylated and dephosphorylated structures of pig heart, GTP-specific succinyl-CoA synthetase.
Fraser ME, James MN, Bridger WA, Wolodko WT
J Mol Biol 299 1325-39 (2000)
PMID: 10873456

Function and Biology Details

Reactions catalysed: 
GTP + succinate + CoA = GDP + phosphate + succinyl-CoA
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127591 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 32.88 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: O19069 (Residues: 38-346; Coverage: 89%)
Gene name: SUCLG1
Sequence domains:
Structure domains:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 396 amino acids
Theoretical weight: 42.84 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P53590 (Residues: 39-433; Coverage: 91%)
Gene name: SUCLG2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
3 modified residues:
Ligand NEP 1 x NEP
Ligand CME 1 x CME
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 86.907Å b: 82.278Å c: 50.017Å
α: 90° β: 104.37° γ: 90°
R-values:
R R work R free
0.162 0.156 0.215
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Impaired TCA cycle flux in mitochondria in skeletal muscle from type 2 diabetic subjects: marker or maker of the diabetic phenotype?
Gaster et al. (2012)
1 more

6 mentions without citation

The many ways that nature has exploited the unusual structural and chemical properties of phosphohistidine for use in proteins.
Kalagiri et al. (2021)
5 more