› 1eus

X-ray diffraction
2Å resolution

SIALIDASE COMPLEXED WITH 2-DEOXY-2,3-DEHYDRO-N-ACETYLNEURAMINIC ACID

Released:
DOI: 10.2210/pdb1eus/pdb
PDB entry 1eus coloured by chain, front view.
PDB entry 1eus coloured by chain, side view.
PDB entry 1eus coloured by chain, top view.
Source organism: Micromonospora viridifaciens
Primary publication:
The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll.
Gaskell A, Crennell S, Taylor G
Structure 3 1197-205 (1995)
PMID: 8591030

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 38.89 KDa
Source organism: Micromonospora viridifaciens
UniProt:
  • Canonical: Q02834 (Residues: 43-407; Coverage: 60%)
Gene name: nedA
Sequence domains: BNR repeat-like domain
Structure domains: Neuraminidase

Ligands and Environments

1 bound ligand:
Ligand DAN 1 x DAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48Å b: 82.86Å c: 84.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 not available

Quick links

Citations

19 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
18 more

10 mentions without citation

A normalized root-mean-square distance for comparing protein three-dimensional structures.
Carugo et al. (2001)
9 more