› 1eys

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF PHOTOSYNTHETIC REACTION CENTER FROM A THERMOPHILIC BACTERIUM, THERMOCHROMATIUM TEPIDUM

Released:
DOI: 10.2210/pdb1eys/pdb
PDB entry 1eys coloured by chain, front view.
PDB entry 1eys coloured by chain, side view.
PDB entry 1eys coloured by chain, top view.
Source organism: Thermochromatium tepidum
Primary publication:
Crystal structures of photosynthetic reaction center and high-potential iron-sulfur protein from Thermochromatium tepidum: thermostability and electron transfer.
Nogi T, Fathir I, Kobayashi M, Nozawa T, Miki K
Proc Natl Acad Sci U S A 97 13561-6 (2000)
PMID: 11095707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162172 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
PHOTOSYNTHETIC REACTION CENTER Chain: C
Molecule details ›
Chain: C
Length: 382 amino acids
Theoretical weight: 40.88 KDa
Source organism: Thermochromatium tepidum
Structure domains: Photosynthetic Reaction Center, subunit C, domain 2
PHOTOSYNTHETIC REACTION CENTER Chain: L
Molecule details ›
Chain: L
Length: 280 amino acids
Theoretical weight: 31.42 KDa
Source organism: Thermochromatium tepidum
Structure domains: Photosystem II protein D1-like
PHOTOSYNTHETIC REACTION CENTER Chain: M
Molecule details ›
Chain: M
Length: 324 amino acids
Theoretical weight: 36.33 KDa
Source organism: Thermochromatium tepidum
Structure domains: Photosystem II protein D1-like
H subunit of photosynthetic reaction center complex Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 28.29 KDa
Source organism: Thermochromatium tepidum
UniProt:
  • Canonical: Q93RD8 (Residues: 1-255; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

9 bound ligands:
Ligand HEM 4 x HEM
Ligand BGL 6 x BGL
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand LDA 1 x LDA
Ligand FE 1 x FE
Ligand MQ8 1 x MQ8
Ligand CRT 1 x CRT
Ligand PEF 1 x PEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 133.299Å b: 196.596Å c: 84.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.287

Quick links

Citations

17 review citations

How lipids and proteins interact in a membrane: a molecular approach.
Lee AG. (2005)
16 more

13 mentions without citation

Membrane protein crystallization in amphiphile phases: practical and theoretical considerations.
Nollert P. (2005)
12 more