› 1f4v

X-ray diffraction
2.22Å resolution

CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM

Released:
DOI: 10.2210/pdb1f4v/pdb
PDB entry 1f4v coloured by chain, front view.
PDB entry 1f4v coloured by chain, side view.
PDB entry 1f4v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of an activated response regulator bound to its target.
Lee SY, Cho HS, Pelton JG, Yan D, Henderson RK, King DS, Huang L, Kustu S, Berry EA, Wemmer DE
Nat Struct Biol 8 52-6 (2001)
PMID: 11135671

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139321 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 128 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Flagellar motor switch protein FliM Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 16 amino acids
Theoretical weight: 1.69 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P06974 (Residues: 1-16; Coverage: 5%)
Gene names: JW1929, b1945, cheC2, fla AII, fla QII, fliM

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand BEF 3 x BEF
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 54.232Å b: 54.233Å c: 347.433Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.258
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Making sense of it all: bacterial chemotaxis.
Wadhams et al. (2004)
18 more

28 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
27 more