› 1f4z

X-ray diffraction
1.8Å resolution

BACTERIORHODOPSIN-M PHOTOINTERMEDIATE STATE OF THE E204Q MUTANT AT 1.8 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1f4z/pdb
PDB entry 1f4z coloured by chain, front view.
PDB entry 1f4z coloured by chain, side view.
PDB entry 1f4z coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin.
Luecke H, Schobert B, Cartailler JP, Richter HT, Rosengarth A, Needleman R, Lanyi JK
J Mol Biol 300 1237-55 (2000)
PMID: 10903866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 24.86 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 18-244; Coverage: 87%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand LI1 13 x LI1
Ligand SQU 1 x SQU
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P63
Unit cell:
a: 60.631Å b: 60.631Å c: 108.156Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.142 0.142 0.203
Expression system: Halobacterium salinarum

Quick links

Citations

25 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
24 more

13 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
12 more