› 1fbn

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF A FIBRILLARIN HOMOLOGUE FROM METHANOCOCCUS JANNASCHII, A HYPERTHERMOPHILE, AT 1.6 A

Released:
DOI: 10.2210/pdb1fbn/pdb
PDB entry 1fbn coloured by chain, front view.
PDB entry 1fbn coloured by chain, side view.
PDB entry 1fbn coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution.
Wang H, Boisvert D, Kim KK, Kim R, Kim SH
EMBO J 19 317-23 (2000)
PMID: 10654930

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.24 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58108 (Residues: 1-230; Coverage: 100%)
Gene names: MJ0697, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 121.407Å b: 43.264Å c: 55.303Å
α: 90° β: 96.99° γ: 90°
R-values:
R R work R free
0.229 0.229 0.248
Expression system: Escherichia coli

Quick links

Citations

29 review citations

The multifunctional nucleolus.
Boisvert et al. (2007)
28 more

7 mentions without citation

AdoMet-dependent methylation, DNA methyltransferases and base flipping.
Cheng et al. (2001)
6 more