› 1fg5

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF BOVINE ALPHA-1,3-GALACTOSYLTRANSFERASE CATALYTIC DOMAIN.

Released:
DOI: 10.2210/pdb1fg5/pdb
PDB entry 1fg5 coloured by chain, front view.
PDB entry 1fg5 coloured by chain, side view.
PDB entry 1fg5 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases.
Gastinel LN, Bignon C, Misra AK, Hindsgaul O, Shaper JH, Joziasse DH
EMBO J 20 638-49 (2001)
PMID: 11179209

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chain: N
Molecule details ›
Chain: N
Length: 310 amino acids
Theoretical weight: 36.91 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 95.67Å b: 95.67Å c: 112.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.298 0.237 0.277
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
18 more

2 mentions without citation

Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases.
Gastinel et al. (2001)
1 more