› 1fka

X-ray diffraction
3.3Å resolution

STRUCTURE OF FUNCTIONALLY ACTIVATED SMALL RIBOSOMAL SUBUNIT AT 3.3 A RESOLUTION

Released:
DOI: 10.2210/pdb1fka/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1fka coloured by chain, front view.
PDB entry 1fka coloured by chain, side view.
PDB entry 1fka coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Structure of functionally activated small ribosomal subunit at 3.3 angstroms resolution.
Schluenzen F, Tocilj A, Zarivach R, Harms J, Gluehmann M, Janell D, Bashan A, Bartels H, Agmon I, Franceschi F, Yonath A
Cell 102 615-23 (2000)
PMID: 11007480

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-289875 (preferred)
Entry contents:
19 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (20 distinct):
30S RIBOSOMAL PROTEIN S2 Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 9.46 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S3 Chain: C
Molecule details ›
Chain: C
Length: 176 amino acids
Theoretical weight: 15 KDa
Source organism: Thermus thermophilus
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
30S RIBOSOMAL PROTEIN S9 Chain: I
30S RIBOSOMAL PROTEIN S10 Chain: J
30S RIBOSOMAL PROTEIN S11 Chain: K
30S RIBOSOMAL PROTEIN S12 Chain: L
30S RIBOSOMAL PROTEIN S13 Chain: M
30S RIBOSOMAL PROTEIN S14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
30S RIBOSOMAL PROTEIN S16 Chain: P
30S RIBOSOMAL PROTEIN S17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
30S RIBOSOMAL PROTEIN S20 Chain: T
16S ribosomal RNA Chain: A

Ligands and Environments

1 bound ligand:
Ligand WO2 7 x WO2
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 406.3Å b: 406.3Å c: 173.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.304 0.304 0.305

Quick links

Citations

128 review citations

Ribosome structure and the mechanism of translation.
Ramakrishnan V. (2002)
127 more

17 mentions without citation

The use of polyoxometalates in protein crystallography - An attempt to widen a well-known bottleneck.
Bijelic et al. (2015)
16 more