› 1fmj

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF MERCURY DERIVATIVE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP

Released:
DOI: 10.2210/pdb1fmj/pdb
PDB entry 1fmj coloured by chain, front view.
PDB entry 1fmj coloured by chain, side view.
PDB entry 1fmj coloured by chain, top view.
Source organism: Spodoptera frugiperda
Primary publication:
A helical lid converts a sulfotransferase to a dehydratase.
Pakhomova S, Kobayashi M, Buck J, Newcomer ME
Nat Struct Biol 8 447-51 (2001)
PMID: 11323722

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 41.56 KDa
Source organism: Spodoptera frugiperda
Expression system: Escherichia coli
UniProt:
  • Canonical: Q26490 (Residues: 1-351; Coverage: 100%)
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand HG 6 x HG
Ligand CA 2 x CA
Ligand A3P 2 x A3P
Ligand RTL 2 x RTL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21
Unit cell:
a: 79.429Å b: 66.183Å c: 83.336Å
α: 90° β: 108.89° γ: 90°
R-values:
R R work R free
0.231 0.231 0.264
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Chemistry of the retinoid (visual) cycle.
Kiser et al. (2014)
3 more

3 mentions without citation

Structural basis for protein phosphatase 1 regulation and specificity.
Peti et al. (2013)
2 more