1fo8

X-ray diffraction
1.4Å resolution

CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I

Released:

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein]
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-150930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.84 KDa
Source organism: Oryctolagus cuniculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27115 (Residues: 105-447; Coverage: 77%)
Gene names: GNT1, MGAT1
Sequence domains: GNT-I family
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 40.382Å b: 82.378Å c: 102.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.185
Expression system: Spodoptera frugiperda