› 1fr9

X-ray diffraction
1.65Å resolution

STRUCTURE OF E. COLI MOBA

Released:
DOI: 10.2210/pdb1fr9/pdb
PDB entry 1fr9 coloured by chain, front view.
PDB entry 1fr9 coloured by chain, side view.
PDB entry 1fr9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of the Escherichia coli MobA protein provides insight into molybdopterin guanine dinucleotide biosynthesis.
Lake MW, Temple CA, Rajagopalan KV, Schindelin H
J Biol Chem 275 40211-7 (2000)
PMID: 10978347

Function and Biology Details

Reaction catalysed: 
GTP + molybdenum cofactor = diphosphate + guanylyl molybdenum cofactor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdenum cofactor guanylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32173 (Residues: 1-194; Coverage: 100%)
Gene names: JW3829, b3857, chlB, mob, mobA, narB
Sequence domains: MobA-like NTP transferase domain
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I422
Unit cell:
a: 124.118Å b: 124.118Å c: 66.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.242
Expression system: Escherichia coli

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Citations

7 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
6 more