› 1fvf

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE ANALYSIS OF NEURONAL SEC1 FROM THE SQUID L. PEALEI

Released:
DOI: 10.2210/pdb1fvf/pdb
PDB entry 1fvf coloured by chain, front view.
PDB entry 1fvf coloured by chain, side view.
PDB entry 1fvf coloured by chain, top view.
Source organism: Doryteuthis pealeii
Primary publication:
Crystal structures of neuronal squid Sec1 implicate inter-domain hinge movement in the release of t-SNAREs.
Bracher A, Weissenhorn W
J Mol Biol 306 7-13 (2001)
PMID: 11178889

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sec1-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 591 amino acids
Theoretical weight: 67.83 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: O62547 (Residues: 1-591; Coverage: 100%)
Sequence domains: Sec1 family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P3121
Unit cell:
a: 118.517Å b: 118.517Å c: 192.621Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.243 0.288
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
Rizo et al. (2012)
7 more

3 mentions without citation

Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*.
Baker et al. (2013)
2 more