› 1fx9

X-ray diffraction
2Å resolution

CARBOXYLIC ESTER HYDROLASE COMPLEX (DIMERIC PLA2 + MJ33 INHIBITOR + SULPHATE IONS)

Released:
DOI: 10.2210/pdb1fx9/pdb
PDB entry 1fx9 coloured by chain, front view.
PDB entry 1fx9 coloured by chain, side view.
PDB entry 1fx9 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Five coplanar anion binding sites on one face of phospholipase A2: relationship to interface binding.
Pan YH, Epstein TM, Jain MK, Bahnson BJ
Biochemistry 40 609-17 (2001)
PMID: 11170377

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, major isoenzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.01 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00592 (Residues: 23-146; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 5 x SO4
Ligand MJI 1 x MJI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P31
Unit cell:
a: 64.987Å b: 64.987Å c: 62.766Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.171 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The interfacial binding surface of phospholipase A2s.
Winget et al. (2006)
1 more

1 mention without citation

Possible roles of S···O and S···N interactions in the functions and evolution of phospholipase A2.
Iwaoka et al. (2006)