› 1fxh

X-ray diffraction
1.97Å resolution

MUTANT OF PENICILLIN ACYLASE IMPAIRED IN CATALYSIS WITH PHENYLACETIC ACID IN THE ACTIVE SITE

Released:
DOI: 10.2210/pdb1fxh/pdb
PDB entry 1fxh coloured by chain, front view.
PDB entry 1fxh coloured by chain, side view.
PDB entry 1fxh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies.
Alkema WB, Hensgens CM, Kroezinga EH, de Vries E, Floris R, van der Laan JM, Dijkstra BW, Janssen DB
Protein Eng 13 857-63 (2000)
PMID: 11239085

Function and Biology Details

Reaction catalysed: 
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139290 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Penicillin G acylase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 27-235; Coverage: 26%)
Gene name: pac
Structure domains:
Penicillin G acylase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 557 amino acids
Theoretical weight: 62.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 290-846; Coverage: 68%)
Gene name: pac
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand PAC 1 x PAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P1
Unit cell:
a: 51.014Å b: 64.084Å c: 64.225Å
α: 72.92° β: 73.91° γ: 73.54°
R-values:
R R work R free
0.181 0.181 0.215
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Penicillin G acylase-based stationary phases: analytical applications.
Calleri et al. (2004)
3 more

1 mention without citation

Excitatory amino acid transporters: roles in glutamatergic neurotransmission.
Divito et al. (2014)