› 1gh4

X-ray diffraction
1.9Å resolution

Structure of the triple mutant (K56M, K120M, K121M) of phospholipase A2

Released:
DOI: 10.2210/pdb1gh4/pdb
PDB entry 1gh4 coloured by chain, front view.
PDB entry 1gh4 coloured by chain, side view.
PDB entry 1gh4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Observation of additional calcium ion in the crystal structure of the triple mutant K56,120,121M of bovine pancreatic phospholipase A2.
Rajakannan V, Yogavel M, Poi MJ, Jeyaprakash AA, Jeyakanthan J, Velmurugan D, Tsai MD, Sekar K
J Mol Biol 324 755-62 (2002)
PMID: 12460575

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00593 (Residues: 23-145; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P2
Unit cell:
a: 39.1Å b: 24.35Å c: 67.11Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.196 0.196 0.259
Expression system: Escherichia coli

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Citations

3 citation in other articles

Modelling studies reveal the importance of the C-terminal inter motif loop of NSP1 as a promising target site for drug discovery and screening of potential phytochemicals to combat SARS-CoV-2.
Prabhu et al. (2021)
2 more

2 mentions without citation

3dSS: 3D structural superposition.
Sumathi et al. (2006)
1 more